In this guide, OnlineTuner.org will teach you how to tune your guitar by ear to standard tuning (EADGBE). We will walk you through identifying the correct pitch for each string and adjusting its tension with the tuning pegs, using three different methods so you can pick the one that suits your ear. By the end you will have a well-tuned guitar and a better understanding of your instrument — and the ability to tune it anywhere, even without a tuner.
Standard guitar tuning, also called E standard, sets the six strings to E2, A2, D3, G3, B3 and E4, from the thickest string to the thinnest. Each string is tuned to a specific note, and just as importantly they must be tuned in relation to one another so the intervals between them stay correct. This is the tuning used as the basis for most music written for the guitar.
One point of confusion is worth clearing up before you start, because it trips up almost every beginner. Guitar strings are numbered from the thinnest to the thickest: the thin E4 is the 1st string and the thick E2 is the 6th string. So the "first string" is the highest-sounding one, not the lowest. Throughout this guide we name strings by their note — E2, A2 and so on — to avoid any ambiguity.

In standard guitar tuning with A4 = 440 Hz, the frequencies of the strings are as follows:
These figures come from the equal-tempered scale with A4 set to 440 Hz. They are the ideal values: the pitch your guitar actually produces will vary slightly with string age, temperature and the accuracy of your tuning device. If you prefer a different reference pitch, our 432 Hz guitar tuner covers the most common alternative.
Before you begin, find a quiet, noise-free spot. The strings of your guitar are anchored at the bridge and the nut, run along the neck and up to the headstock, where they wind around the tuning pegs. Turning a peg changes the tension of that string, and therefore its pitch.

To raise the pitch of a string, turn its peg counterclockwise: this tightens the string and produces a higher note. To lower the pitch, turn the peg clockwise to release tension. Keep plucking the string as you turn so you can hear where you are, and move in small increments — sudden, large adjustments are how strings get broken.
Whenever possible, arrive at the note from below. If a string is sharp, drop it clearly flat and then bring it back up. Coming up to pitch takes up the slack in the peg and the winding, and the string will hold its tuning far better than if you ease down onto the note from above.
Every method below works the same way: you tune one string accurately against an outside reference, then tune the remaining five in relation to it. That reference can be another instrument, a tuning fork, or one of the pitches from our online guitar tuner.
Most players use the thick E2 string for this and work upward, which is the order we follow here. The goal is not only that each string hits its correct pitch, but that they sound right together — that relationship is what makes a guitar sound in tune rather than merely close.
For the rest of this guide we will assume your E2 string is tuned and serving as the reference. Here are three methods, from the most beginner-friendly to the most demanding.
Fretting the 5th fret of a string produces the same note as the next open string along. Play the two together and adjust until they match — when two pitches are close but not identical you will hear a slow wavering, or beat, between them; as they converge the wavering slows and disappears.
The B string is the exception, and it is not an arbitrary one. Five of the six strings sit a perfect fourth apart, which is five semitones — the 5th fret. The gap between G3 and B3 is a major third, only four semitones, so that pairing needs the 4th fret instead. Every guitarist eventually memorises this quirk; knowing why it exists makes it much harder to forget.
This method compares natural harmonics rather than fretted notes. Harmonics ring clean and bell-like, with no finger pressure to pull the note sharp, so small discrepancies are easier to hear. To produce one, rest a fingertip lightly on the string directly above the fret wire, pluck, and lift the finger away at once.
That fourth step is not an oversight. Because G3 and B3 are a major third apart rather than a fourth, the harmonics simply do not line up: the 5th-fret harmonic on the G string rings at 783.99 Hz while the 7th-fret harmonic on the B string rings at 739.99 Hz. Forcing them to match would leave your B string badly out of tune, so the fretted note is the correct tool there.
The harmonic at the 12th fret sounds exactly one octave above the open string, which makes it a quick way to verify a string against itself. It is also the standard test for intonation: fret the 12th fret normally and compare it with the 12th-fret harmonic. If the fretted note is sharper or flatter than the harmonic, your guitar needs its intonation adjusted at the bridge, and no amount of tuning at the pegs will fix that.
Tuning against an open chord is more advanced, but it is the closest test to how the guitar actually gets played. The idea is to adjust the strings so a full chord — typically E major — rings true, working two strings at a time out from your reference string.
| String | Note | Frequency | How to tune it by ear |
|---|---|---|---|
| 6th | E2 | 82.41 Hz | Reference string — tune this one first |
| 5th | A2 | 110.00 Hz | 5th fret of E2, or 5th/7th harmonics |
| 4th | D3 | 146.83 Hz | 5th fret of A2, or 5th/7th harmonics |
| 3rd | G3 | 196.00 Hz | 5th fret of D3, or 5th/7th harmonics |
| 2nd | B3 | 246.94 Hz | 4th fret of G3 — harmonics do not work here |
| 1st | E4 | 329.63 Hz | 5th fret of B3, or 5th/7th harmonics |
If your guitar drifts within minutes of tuning it, the cause is usually mechanical rather than a failure of your ear.
New strings stretch. A fresh set will keep going flat for the first few days and may need retuning several times in one session. Gently pulling each string away from the fretboard speeds this up considerably.
Temperature and humidity move the neck. A guitar brought in from a cold car will drift as it warms. Let the instrument settle before a serious tuning.
The nut can bind. If a string jumps in pitch with a small ping as you tune, it is catching in its slot rather than sliding through. This is very common on guitars with poorly cut nuts and is worth having looked at.
Old strings will not hold pitch. Strings that look dull and feel gritty have lost their elasticity and often cannot be intonated properly at all. Changing them fixes more tuning problems than any technique.
Once you can tune by ear in standard tuning, alternative tunings become much easier, because most are described as changes relative to EADGBE. Drop D only asks you to lower the E2 string by a whole tone, which you can check against the open D3 string an octave above. Half step down moves every string by one semitone and leaves all the relationships in this guide intact, so the same fret numbers still apply.
Open tunings change more. Open G and DADGAD retune several strings so the open instrument sounds a chord, which means the 5th fret pattern no longer holds and each tuning needs its own reference points. If you play another instrument, the same principles carry over — our guide to tuning a bass guitar by ear covers the four-string equivalent, where the 5th fret works on every string with no exceptions.
Tuning by ear is a skill that develops with practice. A few things speed it up:
Listen for the beats, not the notes. Beginners try to judge whether two pitches are the same. It is far easier to listen for the wavering between them and tune until it stops.
Use a tuner as a training tool. Tune by ear first, then check yourself against our online guitar tuner. Seeing how close you were is what trains your ear.
Trust your ears. If a chord sounds wrong even though every string reads as correct, believe the chord. Slight adjustments away from theoretical pitch are common and normal.
Check your work. Play a few open chords in different positions once you have finished. Problems show up in chords long before they show up on single strings.
Maintain your guitar. Clean strings, changed regularly, and a properly set up instrument make tuning dramatically easier. Persistent tuning trouble is often a setup issue rather than a technique issue.
Tuning by ear is a genuinely valuable skill for any musician. It keeps your guitar sounding its best even when there is no tuner within reach, and it teaches you a great deal about how your instrument actually works.